human psgl 1 fc fusion protein  (R&D Systems)

Journal: Journal of Controlled Release

Article Title: Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins

doi: 10.1016/j.jconrel.2010.10.011

Figure Lengend Snippet: Retargeting protein G-pHPMA-coated adenovirus using alternative Fc-bearing ligands targeting murine endothelia. (a) Transduction of murine bEnd3 cells using StrepGpcAdluc retargeted via E-selectin with a rat anti-mouse E-selectin IgG2a monoclonal antibody (RMES mAb). (b) Transduction of murine bEnd3 cells using StrepGpcAdluc retargeted via P-selectin with a chimeric P-selectin Glycoprotein Ligand-1 (PSGL-1)-Fc fusion protein. In each case prior to infection cells were treated with recombinant murine-TNF-α (rmTNF-α, 120 ng/ml) for 5 h at 37 °C. Ad, unmodified adenovirus; pcAd, pHPMA-adenovirus; StrepGpcAd, protein G-pHPMA-adenovirus; RMES-StrepGpcAd, RMES retargeted-protein G-pHPMA-adenovirus; and PSGL-1-Fc-StrepGpcAd, PSGL-1 retargeted-protein G-pHPMA-adenovirus. Results are representative of at least three different experiments. Error bars are ± standard deviation. P values were calculated using a one-way ANOVA (analysis of variance). *P < 0.05, **P < 0.01.

Article Snippet: Recombinant human P-selectin Glycoprotein Ligand-1 (PSGL-1)-/IgG1 Fc fusion protein was purchased from R&D systems (Abingdon, UK).

Techniques: Transduction, Infection, Recombinant, Standard Deviation

Journal: Journal of Controlled Release

Article Title: Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins

doi: 10.1016/j.jconrel.2010.10.011

Figure Lengend Snippet: Pharmacokinetics of PSGL-1-Fc retargeted virus. (a) Blood circulation profile of PSGL-1-Fc-StrepGpcAd (squares) and Adluc (diamonds) in vivo following intravenous administration of 1e10 virus particles. (b) Number of virus genomic copies recovered from HepG2 xenograft tumours following administration of Adluc, StrepGpcAdluc, IgG1-StrepGpcAdluc or PSGL-1-Fc-StrepGpcAdluc. P values were calculated using a one-way ANOVA (analysis of variance). *P < 0.05.

Article Snippet: Recombinant human P-selectin Glycoprotein Ligand-1 (PSGL-1)-/IgG1 Fc fusion protein was purchased from R&D systems (Abingdon, UK).

Techniques: Drug discovery, Virus, In Vivo

Journal: Journal of Controlled Release

Article Title: Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins

doi: 10.1016/j.jconrel.2010.10.011

Figure Lengend Snippet: Targeting endothelial cells in human tumour xenografts by systemic vector administration. Cryostat sections (10 μm) of HepG2 tumour xenografts from mice injected with Adluc or PSGL-1-Fc-StrepGpcAdluc. Photomicrographs were taken using a confocal microscope (Nikon Optiphot-2 upright microscope). (a–c) 20× and (j–l) 60× PSGL-1-Fc-StrepGpcAdluc-infected tumour sections; (d–f) 20× and (m–o) 60× Adluc-infected tumour sections; (g–i) 20× PBS-treated tumour sections. CD31 in red; Firefly luciferase in green; CD31/Firefly luciferase co-localisation (orange/yellow).

Article Snippet: Recombinant human P-selectin Glycoprotein Ligand-1 (PSGL-1)-/IgG1 Fc fusion protein was purchased from R&D systems (Abingdon, UK).

Techniques: Plasmid Preparation, Injection, Microscopy, Infection, Luciferase